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Studium polymorfizmu DNA mrkve
Sejbalová, Darina
This work is focused on theoretical and practical study of genetic diversity in carrots species. The aim of this work was to analyze the DNA polymorphism in Daucus carota L. by using molecular biology methods based on DNA isolation, PCR and electrophoretic separation. A total of 20 samples were analyzed by using 10 microsatellite markers. It was detected a 50 alleles with an average of five alleles per locus and alleles size between 180 and 380 bp. Further were calculated statistical parameters DI, PI and PIC, which confirmed the high value of polymorphism of used SSR markers. Dendrogram was compiled, identifying the relationships between different varieties of carrots. The results were compared with scientific publications dealing with the same or very similar problems.
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Využití molekulárních markerů ve šlechtění mrkve
Sejbalová, Darina
The aim tis work was study of diversity in carrot (Daucus carota), the analysis of the homogeneity of the parent material, the evaluation of the success of the crossing, and the resolution of the sterile and fertile genotypes of the samples used in crossing. Four hybrid carrot varieties and 12 lines were analyzed, four of which were sterile (CMS), with ten SSR markers and six CMS markers. Based on the calculated statistical values of DI, PI and PIC, the markers were highly variable. A total of 45 alleles were detected, with a mean of 4.5 alleles per locus and 4 zero alleles. The size of the allele ranged between 180 and 380 bp. The success rate of the crossing was evaluated, when the crossing of the Koloseum variety was evaluated as the most successful. Four SSR markers have been recommended as suitable for further study of the diversity and success rate of carrot crosses, namely GSSR-6, GSSR-16, GSSR-91 and GSSR-9. CMS markers successfully identified the sterile and fertile lines at one crossing (variety Koloseum) by Atp8 marker.
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Analýza genetické variability konopí pomocí DNA markerů
Balgová, Barbora
This thesis is focused on analysis of genetic variability of Cannabis sativa L. and sequencing of the candidate genome sequences in selected varieties of Cannabis. In total there were 28 genotypes of hemp were analyzed with 23 microsatellite markers.107 alleles were found whose size ranged from 100 to 360 bp. Uniform marker was detected (CAN1660). The diversity index (DI), the polymorphic information content (PIC) and the probability of identity (PI) were calculated for every microsatellite marker. Similarity dendrogram was constructed on base of statistical evaluation. The specific primer for the patrial sequences of cannabidiolic acid synthase (CBDA) gene and the specific complete for the sequences of tethrahydrocannabinolic acid synthase (THCA) gene were used for the sequences study. Gained sequences were compared by BLAST. Most sequences had 100% match with sequences in the available databases. Single nucleotide polymorphisms were found in the sequences of CBDA synthase. One sequences that did not belong to the genus Cannabis was detected. All obtained sequences will be inserted into the NCBI database and access number will be assigned.
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Methodology for detecting the presence of cranberries and lingonberries in processed products using molecular SSR markers: methodology for practice
Kučera, Ladislav ; Ovesná, Jaroslava
The subject of the methodology is a procedure for diagnosing the presence of lingonberries (Vaccinium vitis-idaea L.) and cranberries (Vaccinum macrocarpon L.) in processed products such as tea or fruit ingredients of many food products (jams, jelly, purree, tea). The methodology exploit DNA sequences that are typical for each plant species. Thus, molecular DNA markers that characterize the plant species by SSRs (single sequence repeats) are used. The methodology describes the extraction of DNA from the assumed matrix, the use of selected SSR markers and the analytical data evaluation procedure. The method can be used in any solid equipped molecular-genetic laboratory. The method is applicable for the characterization of DNA isolated from any part of the plant, fruit, and derived food or food supplements. Method describes the procedure and necessary equipment to perform the analysis. Protocol recommends how to evaluate the results.
Fulltext:  PDF
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Studium polymorfizmu DNA mrkve
Sejbalová, Darina
This work is focused on theoretical and practical study of genetic diversity in carrots species. The aim of this work was to analyze the DNA polymorphism in Daucus carota L. by using molecular biology methods based on DNA isolation, PCR and electrophoretic separation. A total of 20 samples were analyzed by using 10 microsatellite markers. It was detected a 50 alleles with an average of five alleles per locus and alleles size between 180 and 380 bp. Further were calculated statistical parameters DI, PI and PIC, which confirmed the high value of polymorphism of used SSR markers. Dendrogram was compiled, identifying the relationships between different varieties of carrots. The results were compared with scientific publications dealing with the same or very similar problems.
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